DNA profiling of disputed chilli samples (Capsicum annum) using ISSR-PCR and FISSR-PCR marker assays
نویسندگان
چکیده
منابع مشابه
Quantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
متن کاملSpecies Specific DNA Profiling Mycobacterial Genomes Using Polymerase Chain Reaction with Single Universal Primer (UP-PCR)
Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...
متن کاملPreparation of DNA from numerous individual microscopic organisms for PCR-based assays of environmental samples.
Polymerase chain reaction (PCR)based assays have been developed to identify microscopic organisms that cause disease (8) or have an environmental impact (1). In both cases, DNA is extracted from pooled individuals. The pooled sample has typically been created for bacteria by culturing and for disease vectors by taking advantage of their reproduction in the host. PCRbased assays are being develo...
متن کاملParallel picoliter rt-PCR assays using microfluidics.
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel singl...
متن کاملMycotic and aflatoxin contamination in Myristica fragrans seeds (nutmeg) and Capsicum annum (chilli), packaged in Italy and commercialized worldwide
Aflatoxins are secondary metabolites of moulds known to be carcinogenic for humans, and therefore should not be ingested in high doses. This study aimed to determine the level of mould and aflatoxin contamination in dehydrated chilli and nutmeg imported from India and Indonesia, respectively, packaged in Italy, and commercialized worldwide. We tested 63 samples of chilli (22 sanitized through h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Forensic Science International
سال: 2001
ISSN: 0379-0738
DOI: 10.1016/s0379-0738(00)00350-9